Insights in the biocatalyzed hydrolysis, esterification and transesterification of waste cooking oil with a vegetable lipase

The present contribution investigates the biocatalytic performance of lipase obtained from latex native plant known as Araujia sericifera (ASL) in hydrolysis followed by esterification released free fatty acids and transesterification glycerides sunflower waste cooking oil WCO. A specific enzymatic activity 719.05 ?mol mg?1 h?1 (60 % conversion triglycerides towards acids) was WCO with 0.05 biocatalyst (typically, 10.0 g 5.0 mg enzyme) 50 w/w water added 30 min reaction at 25 °C homogeneous type system. has capacity to catalyze mainly monoglycerides diglycerides a lesser extent. FFAs remaining reacted tandem addition short chain alcohols immediately after (without more enzyme media). Above 90 methanol, ethanol, n-propanol n-butanol contents low 1:0.2 oil: alcohol molar ratio °C. (about 20 %) also observed although no further detected even under great excess alcohol.